Bug-Wash Jeans
Scientists Create Bacteria That Can Dye Jeans Blue

By Amanda Onion


Oct. 15  There were stonewashed jeans, then acidwashed jeans. Now
scientists have developed jeans colored by vats of active bacteria.

Bacteria have been adapted before to help create the indigo dye used to
color jeans. But just as the plant-derived dyes carry a trace of red
pigment, the bacteria-produced dye also contained some of the
unfashionable red hue. Power and his colleagues recently found a way to
tweak the organism Escherichia coli to eliminate the trace of red in its
pigment, leaving a deep true blue.
"We created a natural ation process that produces exactly what the fashion
industry describes as the indigo look," says Scott Power, a research
fellow at Genencor International in Palo Alto, Calif.

The bacteria, itself, doesn't turn the dye blue. Instead it converts sugar
(usually corn syrup) into a reddish amino acid called tryptophan. Genencor
spliced a gene into the bacteria to eliminate the red tones and create a
substance called indoxl, which spontaneously turns blue when exposed to
air. They call the color "bioindigo."

"It's a lot like producing beer," said Power.

A 'Green' Way to Make Indigo

The research was done using funding from Levi Strauss, and Genencor
produced 400,000 square yards of bacteria-dyed jean material. But Levi
Strauss indicates they won't be buying bioindigo jeans any time soon.

"Most indigo is now manufactured in China where environmental regulations
aren't as steep," explains Jack Huttner, vice president for corporate
communications and public affairs at Genencor. "Our process is cost
effective, but it's not cost competitive to materials coming from China."

Indigo, the deep blue dye most commonly applied to jeans, was first
derived from plants such as woad and dyer's knotweed. Levi Strauss used
the dye in the 19th century in his tear-free clothes designed for farmers
and gold miners. By the early 1900s, manufacturers began using a chemical
process that drew dye from coal or oil, but that process can cause
environmental damage.

Escherichia coli produces the dye with almost no environmental side
effects and Power holds out hope that an increasingly environmentally
conscious consumer might someday pay for the bacteria-dyed jeans in the
future.

"When the playing fields become more level I think environmentally
produced clothes will have a better chance," he says.

Bug-Designed

Bacteria already leave their mark in other parts of the jean-making
process.

Most jean companies, including Levis and Wrangler no longer use stones to
make their stonewashed jeans. Instead the material is washed with
bacteria-made enzymes that soften and fade the material with the same
effect. Environmentalists were happy to see traditional stonewashing take
backseat to enzymes since the old method required lots of energy to
operate the washers and drained pumice stone resources from mines.

Bacteria-made enzymes are also used when bleaching genes to faded hues.
Rather than using water to flush out bleach from the jeans, manufacturers
first introduce bleach-eating enzymes. The enzymes consume most of the
bleach in the jeans so minimal amounts are leached into water drainage
systems.

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